P0205
Genotyping Platforms for the Validation of SNPs in T. aestivum
Wheat breeding has traditionally used phenotyping methods to select desired traits such as enhanced yield, suitability to a particular climate and resistance to biotic or abiotic stresses. The current rate of yield improvement will not be sufficient to feed the ever increasing global population. As such there is a need to assist conventional and time consuming phenotypic breeding methods.
Molecular markers are a tool used to analyse the plant genome and may also be used to assist plant breeding by facilitating the identification of genes associated with desirable traits. Breeders can use markers to follow the genes through a population including those for recessive traits or traits with complex inheritance patterns and hence reduce the time required for breeding. Single Nucleotide Polymorphisms (SNPs) are popular markers as they are highly abundant in the genome and well suited for use on current high-throughput genotyping platforms. We have identified and validated over 5,000 SNP markers on 169 wheat lines and over 4000 of these have been assigned a genetic map location. These markers have been validated using several recently developed high-throughput genotyping platforms, enabling comparisons of their effectiveness in screening wheat SNPs. The validated markers and screening data are made publicly available through our web-based database to assist wheat breeders and researchers worldwide.