Genotyping By Sequencing (GBS) of Citrus: Genetic Diversity Analyses, Linkage Map Construction and QTL Mapping

The objective of this work was the development and application of DArT marker platform in combination with Illumina platform short read sequencing aiming to study the relationships among a group of 94 cultivars, species and hybrids of citrus, the construction of genetic linkage maps and QTL mapping of agronomic traits of an intergeneric hybrids population of citrus. The progeny of Citrus sunki and Poncirus trifoliata was evaluated for various horticultural traits of the scion as harvest season, scion height, yield, juice quality, and drought tolerance when the hybrids were used as rootstocks and for characteristics of the hybrids as number of seeds per fruit, number of fruit per plant, plant height and disease resistance. The map consisted of DArT, SSR, RAPD and TRAP markers. Twenty three QTLs were identified for important agronomic traits.