P0801
A Genomic Approach to Understanding Fruit-Pedicel Abscission in Sweet Cherry
A Genomic Approach to Understanding Fruit-Pedicel Abscission in Sweet Cherry
Date: Monday, January 14, 2013
Room: Grand Exhibit Hall
Sweet cherries are valued at $700 million in the US market. Historically, sweet cherries have been harvested by hand. Due to a shrinking workforce and rising labor costs, the sweet cherry industry would greatly be benefited by an option to mechanically harvest the fruit. We have taken a genetic approach to focus on the trait that could make this goal a reality. Sweet cherries are non-climacteric; however, there is an ethylene-induced abscission pathway operative in some genotypes that when activated results in the development of a clearly defined abscission zone between the fruit and the pedicel. In order to identify the genetic underpinnings of this process, we performed a time course transcriptome analysis of fruit-pedicel abscission zone in presence and absence of ethephon in (1) ethephon unresponsive Chelan genotype, (2) ethephon responsive Bing genotype, and (3) and auto-abscising Skeena genotype. These varieties are representative of the range of genes and alleles participating in the formation of fruit-pedicel abscission zone in sweet cherry genotypes. These collective RNA-seq data has been assembled into a provisional representative transcriptome. The alleles identified by this transcriptomic analysis can be utilized by sweet cherry breeding programs for development of varieties that exhibit the desired level of fruit-pedicel retention.
