Cloning and Expression of Four Salt-Tolerance Genes of Gossypium hirsutum

As one of main abiotic stresses in nature, salt stress with high concentration of saline ions in soil does remarkable harm to plants, and seriously affects the growth and development of plants. Therefore, it is particularly important to excavate salt-tolerant genes of cotton, to innovate cotton salt-tolerance germplasm in order to lay the foundation for salt-tolerant cotton breeding. In this study, four salt-tolerance related genes, H+-pyrophosphatase gene (GhVP), S-adenosylmethionine synthetase gene (GhSAMS), Thioredoxin gene (GhTrx) and Glutathione Reductase gene (GhGR), were cloned from the salt-tolerance material on Gossypium hirsutum. The bioinformatics analysis was conducted, that GhVP protein had the similarity of 90% with Arabidopsis and of 93% with tobacco and that GhSAMS protein had the similarity of 91%, 93%, 93% with Arabidopsis, S. salsa, O.Sativa, respectively. Under salt stress, GhVP provided proton driving force for Na+/H+ antiproton, and promoted distribution of Na+ in cell, in order to eliminate Na+ infection. GhSAMS enhanced the cell-to-cell pathway for water transport and reduced ions up-taking, and improved the adaptations of whole cotton under salt stress.