Plant and Animal Genome XXI Conference

January 11 - 16, 2013

Integrated DNA Technologies

Gene cloning using PCR is a well-established and effective technique when constructing genes using native DNA sequences. However, when modifying or assembling novel genes, traditional PCR methods can be very inefficient. This often leads researchers to either assemble the new sequence de novo using oligonucleotides or they turn to a gene synthesis service provider to generate such constructs. Integrated DNA Technologies (IDT) now offers in addition to a standard gene synthesis service: a novel, rapid, and reliable method to build and clone the genes you need at a fraction of the cost of full gene synthesis services, using gBlocks™ Gene fragments - double-stranded, sequence-verified DNA blocks up to 500 base pairs. Their high sequence fidelity and rapid delivery time make gBlocks Gene Fragments ideal for a range of biology applications, including easy assembly of multiple gene fragments to reliably generate larger gene constructs. The goal of this workshop is to enable the bench scientist to use gBlocks to easily and rapidly construct novel DNA sequences using the Gibson Assembly Method. Products, cloning protocols, and applications will be discussed in addition to troubleshooting recommendations.