Construction of Linkage Map through Genotyping-By-Sequencing (GBS) in Lentil

GBS is a new approach for detecting SNPs in large segregating populations. It is a fast and inexpensive system for studying diversity and mapping of molecular marker or trait. SNPs are chosen because of the abundance and stability in the genome. By using SNP markers it is possible to generate large number of markers within a short distance along chromosomes that are evenly distributed throughout the genome.

Lentil is an important crop cultivates in wide area of the world because of its role in management of soil fertility and importance in both human diet and animal feed. It is a self-pollinating, diploid (2n=2x=14 chromosomes) crop, with a haploid genome size of 4,063 Mbp. In this study, 101 individuals developed from a Precoz X WA8649041 cross, with single-seed descent are genotyped and SNPs are detected by GBS technology. The linkage map is constructed by using JoinMap4 software. Five hundred and nineteen out of 838 SNPs (%61) were mapped on 12 linkage groups. The 519 markers covered 539.5 cM provided an average marker density of 1 marker per 1.03 cM. Linkage group sizes were ranging from 92.9 cM to 11.3 cM with a mean length of 44.9 cM and 43 markers per linkage group. This study provides the first results about GBS in lentil. The availability of large amount of data generated in this study will be useful for genetic characterization, creating high-density linkage map, genome-wide analyses of molecular variation and genome-based QTL analysis.