Charles H. Langley
,
Department of Evolution and Ecology, University of California, Davis, Davis, CA
Jill L. Wegrzyn
,
Department of Plant Sciences, Univ.of California-Davis, Davis, CA
Kristian Stevens
,
Department of Evolution and Ecology, University of California, Davis, Davis, CA
Aleksey Zimin
,
Institute for Physical Science and Technology, University of Maryland, College Park, MD
Daniela Puiu
,
Johns Hopkins University, School of Medicine, Baltimore, MD
Marc Crepeau
,
Department of Evolution and Ecology, University of California, Davis, Davis, CA
Charis Cardeno
,
University of California, Davis, Davis, CA
Maxim Koriabine
,
Children's Hospital Oakland Research Institute, Oakland, CA
Ann Holtz-Morris
,
Children's Hospital Oakland Research Institute, Oakland, CA
John Liechty
,
Department of Plant Sciences, University of California, Davis, Davis, CA
Pedro J Martinez-Garcia
,
Department of Plant Sciences University of California, Davis, CA
Hans Vasquez-Gross
,
University of California Davis, Davis, CA
Brian Y. Lin
,
Department of Plant Sciences, Univ. of California-Davis, Davis, CA
L. L. Zieve
,
Department of Plant Sciences, University of California, Davis, CA
Matt Dougherty
,
University of California, Davis, Davis, CA
S. Fuentes-Soriano
,
Department of Biology, Indiana University, Bloomington, IN
L. Wu
,
National Center for Genome Analysis Support, Indiana University, Bloomington, IN
D. Gilbert
,
Department of Biology, Indiana University, Bloomington, IN
G. Marçais
,
Institute for Physical Sciences and Technology (IPST), University of Maryland, College Park, MD
M. Roberts
,
.Institute for Physical Sciences and Technology (IPST), University of Maryland, College Park, MD
C. Holt
,
Department of Human Genetics, University of Utah, Salt Lake City, UT
M. Yandell
,
Department of Human Genetics, University of Utah, Salt Lake City, UT
J.M. Davis
,
School of Forest Resources and Conservation, Gainesville, FL
K. Smith
,
Southern Institute of Forest Genetics, USDA Forest Service, Saucier, MS
J.F.D. Dean
,
Warnell School of Forestry and Natural Resources, University of Georgia, Athens, GA
W.W. Lorenz
,
Warnell School of Forestry and Natural Resources, University of Georgia, Athens, GA
R.W. Whetten
,
Department of Forestry and Environmental Resources, North Carolina State University, Raleigh, NC
R. Sederoff
,
Department of Forestry and Environmental Resources, North Carolina State University, Raleigh, NC
Nicholas Wheeler
,
Pennsylvania State University, Centralia, WA
Pat McGuire
,
Department of Plant Sciences, University of California, Davis, Davis, CA
Dorrie Main
,
Washington State University, Pullman, WA
Keithanne Mockaitis
,
Indiana University, Bloomington, IN
Carol Loopstra
,
Texas A&M University, College Station, TX
Pieter J. deJong
,
Children's Hospital Oakland Research Institute, Oakland, CA
James A Yorke
,
University of Maryland, College Park, MD
Steven L Salzberg
,
Johns Hopkins University, School of Medicine, Baltimore, MD
David Neale
,
Dept. Plant Sciences University of California Davis, Davis, CA
The size and complexity of conifer genomes (c. 20 to 40 Gb, 2n = 24) pose a formidable challenge to full genome sequencing and assembly. We developed a new approach to sequence the genome of loblolly pine (Pinus taeda L.). It leveraged unique aspects of pine reproductive biology and deployed recents advances in genome assembly methodology. We used whole genome shotgun sequencing based primarily on next generation sequence generated from a single haploid seed megagametophyte (conifer seed endosperm) from the loblolly pine tree genotype 20-1010 used in industrial forest tree breeding and research. The resulting sequence and assembly led to a draft genome spanning 23.2 billion base pairs and containing 20.1 billion sequenced bases with an N50 scaffold size of 66.9 Kbp.
