W563
Genetic Mapping of Scaffolds By Whole Genome Shotgun Sequencing

Date: Saturday, January 11, 2014
Time: 11:10 AM
Room: Pacific Salon 6-7 (2nd Floor)
Pedro J Martinez-Garcia , Department of Plant Sciences University of California, Davis, CA
Kristian Stevens , Department of Evolution and Ecology, University of California, Davis, Davis, CA
Marc Crepeau , Department of Evolution and Ecology, University of California, Davis, Davis, CA
John Liechty , Department of Plant Sciences, University of California, Davis, Davis, CA
Charis Cardeno , University of California, Davis, Davis, CA
Jill L. Wegrzyn , Department of Plant Sciences, Univ.of California-Davis, Davis, CA
Charles H. Langley , Department of Evolution and Ecology, University of California, Davis, Davis, CA
David Neale , Dept. Plant Sciences University of California Davis, Davis, CA
We elaborate on an approach we call Haplotyping by Sequencing (HBS) for annotating recombination in loblolly pine by whole genome shotgun sequencing of haploid megagametophytes. We have performed DNA extractions on over 600 megagametophytes from 20‐1010 using a commercial plant DNA extraction kit. Light shotgun sequencing of these haploid megagametophytes was carried out. We report on progress made in annotating recombination, constructing a genetic map,  and ordering, orienting and validating whole genome shotgun scaffolds of the loblolly pine reference genome.
Back to: Pine Genome Reference Sequence
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