Automated RNA Sample QC Using the Agilent 2200 TapeStation System

The Agilent 2200 TapeStation system provides a flexible solution for automated analysis of up to 96 samples using pre-packaged reagents and minimal manual handling. Here we present a new assay – the RNA ScreenTape assay - to enable robust quantification and quality analysis of Total RNA samples from both eukaryotic and prokaryotic sources from 100 pg/µl to 500 ng/µl. The new assay additionally benefits from the ability to provide separation of contaminant genomic DNA allowing more accurate purity assessment of sample material. This study compares the performance of the RNA ScreenTape and High Sensitivity RNA ScreenTape assays against the market leading Agilent 2100 Bioanalyzer and NanoDrop for RNA quality and quantity determination. We conclude that the new RNA ScreenTape and High Sensitivity assays provide correlated data to these existing technologies, as well as exceeding these technologies in terms of flexibility or quality of data generated.