Automated Assessment of Next Generation Sequencing Library Preparation Workflow for Quality and Quantity Using the Agilent 2200 TapeStation System

Quality and quantity assessment of the Next Generation Sequencing (NGS) library manufacture is critical in ensuring successful sequencing results. The Agilent Genomic DNA ScreenTape and the new D1000 ScreenTape assays have been developed to provide a reproducible QC method for analysing samples in this library preparation workflow. The Genomic DNA ScreenTape assay combined with the Agilent 2200 TapeStation system, automates the assessment of the starting genomic DNA with sample volume as low as 1µl and producing digital results in less than 2 minutes per sample. The D1000 ScreenTape assay provides a QC platform for analysing the library construction throughout the workflow by automating electrophoretic separation, sizing and quantification. In addition, the ability to overlap and compare electropherograms within the 2200 TapeStation Analysis software enables the discrimination of sample quality during the workflow. We present data that shows application of the Agilent 2200 TapeStation system for quantification and quality assessment of NGS library preparation workflow from starting material QC to final library quality and quantity determination for the Agilent SureSelect enrichment protocol.