Tracking the Evolutionary Fates of Cis-Regulatory Elements Surrounding Duplicated Genes through the Brassica lineages (Mapping Over)

The Brassica lineages features a recent hexaploidy following divergence from the arabidopsis lineage. The evolutionary fate of genes duplicated in this hexaploidy can be tracked through the sequenced genomes of the Brassica lineages. At present, one or more  genome sequences are available for Brassica rapa,Brassica napus, Brassica oleraceae and Brassica nigra. Using synteny to define orthologous regions, the impact of post-hexaploidy fractionation can be determined within these genomes. Using our Mapping Over Pipeline, we have determined the fates of potential cis-regulatory elements in the vicinity of duplicated genes, and compared them between the various Brassica polyploids.  Subfunctionalizations can be readily distinguished from nonfuctionalizations, and these mutational outcomes have been used to predict a gene's subgenomes or origin, mRNA levels and something about the organ-specific patterns of expression.