W339Maize Meiotic Recombination: Gene Expression and Its Regulation
Date: Tuesday, January 14, 2014
Time: 5:10 PM
Room: Royal Palm Salon 1,2,3
Joann Mudge
,
National Center for Genome Resources (NCGR), Santa Fe, NM
Stefanie Dukowic-Schulze
,
University of Minnesota, St. Paul, MN
Anitha Sundararajan
,
National Center for Genome Resources (NCGR), Santa Fe, NM
Thiru Ramaraj
,
National Center for Genome Resources, Santa Fe, NM
Andrew D. Farmer
,
National Center for Genome Resources (NCGR), Santa Fe, NM
Minghui Wang
,
Bioinformatics Facility, Cornell University, Ithaca, NY
Qi Sun
,
Institute for Genomic Diversity, Cornell University, Ithaca, NY
Farhad Ghavami
,
Dept of Plant Pathology, University of Minnesota, Saint Paul, MN
Yan He
,
Cornell University and China Agricultural University, Ithaca, NY
Jaroslaw Pillardy
,
Cornell University, Ithaca, NY
Shahryar Kianian
,
USDA-ARS Cereal Disease Laboratory
Wojtek Pawlowski
,
Cornell University, Ithaca, NY
Changbin Chen
,
University of Minnesota, St. Paul, MN
Meiocytes are the cells where meiosis occurs and the instrument of genetic recombination. Recombination, as the main source of genetic variation, is crucial to a population’s survival, enabling adaptation to changing conditions and purging deleterious mutations from the gene pool. It is also a main driving force behind plant improvement efforts.
Recombination is a major determinant of an organism’s genome architecture and its phenotypic translation. The distribution of these events in genomic space has a critical impact on a genomic region’s plasticity and/or stability, and, consequently on the organism’s ability to adapt and survive.
While recombination has such an important impact on regional genomic architecture, it is also likely that local genome features dictate recombination sites and hotspots. As part of a larger project exploring the genomic landscape of recombination in maize, here we report specifically on the transcriptional landscape of maize meiotic cells, including exploring the regulation of transcription.
We looked at mRNA and smRNA expression patterns in prophase I meiocytes as well as anthers and seedlings. In addition, we compared these patterns to H3K4me3 histone methylation patterns and recombinational hotspots to better understand meiosis and recombination.
