P841
Comparing Hypothalamic Transcriptomes Between Modern and Legacy Chicken Lines

Date: Monday, January 13, 2014
Room: Grand Exhibit Hall
Lakshmi Praveena Kamineni , University of Delaware, Newark, DE
Susan J. Lamont , Iowa State University, Ames, IA
Max F. Rothschild , Iowa State University, Ames, IA
Michael Persia , Iowa State University, Ames, IA
Chris Ashwell , North Carolina State University, Raleigh, NC
Carl J. Schmidt , University of Delaware, Newark, DE
Broiler chickens have evolved under human selection for rapid growth and high muscle yield. The hypothalamus plays a central roll in maintaining energy balance, and it is reasonable to hypothesize that selective pressures on broiler lines may have affected hypothalamic gene expression patterns. To explore this hypothesis, we have compared post-hatch hypothalamic transcriptomes between a modern broiler line, Ross 708, and a legacy line that grows with properties of meat birds from the 1950s. In addition, a portion of the birds were raised under heat stress conditions designed to mimic heat waves. At days 7,21,28 and 42 post hatch, birds were humanely euthanized, and hypothalami isolated and quick frozen in liquid nitrogen. Transcriptome libraries were prepared using Illumina TruSeq kits, sequenced on an Illumina HiSeq2500 and analyzed using the Tuxedo suite. Comparisons between control and heat stress transcriptome will be presented.
Back to: Genes & Pathways (Gene Expression, Proteomics, Metabolomics): Cellular Processes and Regulatory Networks
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