P843
Comparison of Jejunal Gene Expression Beteween Modern and Legacy Broiler Lines

Date: Monday, January 13, 2014
Room: Grand Exhibit Hall
Antonio Pinto Garcia , University of Delaware, Newark, DE
Matheus Reis , University of Delaware, Newark, DE
Susan J. Lamont , Iowa State University, Ames, IA
Max F. Rothschild , Iowa State University, Ames, IA
Michael Persia , Iowa State University, Ames, IA
Chris Ashwell , North Carolina State University, Raleigh, NC
Carl J. Schmidt , University of Delaware, Newark, DE
The modern broiler chicken has evolved under human selection for rapid growth, increase muscle yield and high feed efficiency.   One morphological difference between modern and legacy broilers is that modern broilers have longer jejuna. Given the role of the jejunum in nutrient absorption, lengthening may have played a role in improved feed efficiency.  To determine if changes in gene expression patterns may have also affected feed efficiency, we have constructed transcriptome libraries to jejuna isolated from a modern broiler (Ross 708) and a legacy line (Illinois). These tissues were obtained at 6 and 21 days post hatch to explore possible temporal changes in gene expression patterns. The libraries were prepared were using Illumina TruSeq kits, sequenced on an Illumina HiSeq2500, processed using the Tuxedo suite and analyzed with JMP.  Comparisons of jejuna gene expression patterns between the Ross and Illinois lines will be presented.