Genome-Wide Association Study for Canine Hip Dysplasia in Multiple Breeds

Canine hip dysplasia (CHD) is the most common complex orthopedic trait in medium and large breed dogs with incidences ranging from less than 10 to over 70% across pure breeds. In this study, the diagnosis is confirmed by pelvic radiography but initially made clinically. Collected on the ventrodorsal extended-hip radiograph, the Norberg angle ranges from excellent hip conformation to severe hip dysplasia with secondary osteoarthritis. A genome-wide association study (GWAS) of CHD was performed by using ~160,000 single nucleotide polymorphisms (SNPs) in a population (n₌872) with multiple breeds including Labrador retriever, German shepherd, Golden retriever and English setter. Principal component and kinship matrix were employed to correct population stratification. Multiple susceptibility SNPs have been identified, including the ones close to previous reports and the one on chromosome 21 near the ANO5 gene that is causative for gnathodiaphyseal dyplasia in human. Our results may provide further insight into the CHD and other related complex disease in canine.