P0452Molecular Characterization of Salmonella Enterica Recovered from Major Food of Animal Origin Collected from Saudi Arabia
Salmonella enterica serovar Enteritidis considered being the most predominant serovar in poultry and eggs. Therefore, S. Enteritidis has been targeted by a number of control programs. The present study is aimed to investigate Salmonella Enteritidis in chicken related samples collected from Kindom of Saudi Arabia using Polymerase Chain Reaction (PCR) and sefb gene specific primers specific for S. Enteritidis. During the summer of 2012, a total of 1100 chicken related samples (450 cloacal swabs, 400 chicken eggs and 250 chicken meats) were collected from 12 poultry farms located in different governorates. The collected samples were identified by standard bacteriological methods as well as PCR using selective broth culture, Rappaport- Vassiliadis (RV) using specific primer for S. Enteritidis. The highest rate of isolation of S. Enteritidis 4.44% was obtained from cloacal swabs and chicken eggs (4%), followed by chicken meats 2%. PCR using selective broth culture (RV) and specific primer for S. Enteritidis could detect all the bacteriolgically positive samples, in addition, to 5 cloacal samples (1.11%) previously identified as negative samples with bacteriological examination. The PCR-RV using primers specific for S. Enteritidis could detect more positive samples of S. Enteritidis than conventional methods for rapid detection of foodborne pathogens.
Key words: Salmonella enterica, PCR-RV, Chicken related samples, S. Enteritidis.