Protein Microarray Platform for Pathogen Effectors Studies and Beyond

Protein microarray technology has emerged as a powerful approach for the study of hundreds or thousands of proteins simultaneously. Since the majority of pathogen effector proteins lack discernable enzymatic or biochemical functions, large-scale characterization of these proteins will depend on the identification of their host targets. Therefore, we are developing plant protein microarrays and pathogen effector microarrays for the comprehensive biochemical and functional analysis of pathogen effectors. We will discuss our efforts towards application of these protein microarrays to understand effector biology.