P1081
Evolution of Panax ginseng Revealed by Genome Sequencing and Chloroplast Genome Diversity

Date: Monday, January 12, 2015
Room:
Tae-Jin Yang , Seoul National University, Seoul, South Korea
Nam-Hoon Kim , Seoul National University, Seoul, South Korea
Woojong Jang , Seoul National University, Seoul, South Korea
Junki Lee , Seoul National University, Seoul, South Korea
Beom-Soon Choi , PHYZEN Genomics Institute, Seoul, South Korea
Kyunghee Kim , Seoul National University, Seoul, South Korea
Nomar Espinosa Waminal , Seoul National University, Seoul, South Korea
Hong-Il Choi , Korea Atomic Energy Reserach Institute, Jeongeup-si, South Korea
Hyun-Seung Park , Seoul National University, Seoul, South Korea
Jonghoon Lee , Seoul National University, Seoul, South Korea
Hyun Oh Lee , Seoul National University, Seoul, South Korea
HyunJoo Lee , Seoul National University, Seoul, South Korea
Ho Jun Joh , Seoul National University, Seoul, South Korea
Jee Young Park , Seoul National University, Seoul, South Korea
Sampath Perumal , Seoul National University, Seoul, South Korea
Murukarthick Jayakodi , Seoul National University, Seoul, South Korea
Yun Sun Lee , Seoul National University, Seoul, South Korea
Yeisoo Yu , Arizona Genomics Institute, University of Arizona, Tucson, AZ
Hyun Hee Kim , Plant Biotechnology Institute, Sahmyook University, Seoul, South Korea
We are at finishing phase for building a high quality reference genome sequence of Panax ginseng using a Korean elite ginseng cultivar Chunpoong. We also do large scale transcriptome analysis in association with metabolome analysis to understand the important metabolic pathway of ginseng. We have generated more than 600 Gbp of paired-end reads which is equal to over 180x genome coverage and seven long mate pair libraries including 3, 5, 10, 20, and 40 kbp libraries. Assembly show a total of 3.1 Gbp scaffolds with 600 kbp at N50 criteria. We will reveal the genome annotation, the allotetraploid nature of sequence, molecular cytogenetic characterization, genetic mapping, and transcriptome analysis. We also show the evolution process by sequence comparison of complete chloroplast genome sequences of five Panax speciesand eight related genus. We also show diversity mong chloroplast genome sequences of 12 ginseng accessions and their utility for barcoding of Panax genus and ginseng cultivars.

Acknowledgement - This work was supported by the grant funded by Next-Generation BioGreen21 Program (No.PJ008202), Rural Development Administration, Rupublic of Korea.

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