High PCR Efficiency Using FASTMAG DNA Extraction Kit

Various DNA extraction methods yield high quantities of DNA but suboptimal DNA quality for quantitative Real Time PCR (qPCR) applications. Here we present qPCR data using the magnetic silica-based DNA extraction kit, FASTMAG, showing that DNA purified from animal tissue, grains and food samples is superior in quality using the FASTMAG kit to that obtained with other commercial magnetic silica-based DNA extraction kits.  The most consistent Relative PCR Efficiency (RPE), a measure of suitability of purified DNA for qPCR, was observed with DNA purified using the FASTMAG kit.

 The FASTMAG kit does not require organic extraction or ethanol precipitation and is suitable for many downstream applications. In addition, the FASTMAG DNA isolation procedure is simple, economical and rapid. For high throughput DNA extraction, the FASTMAG kit is currently available in 96-well plate format. One single DNA extraction kit can be used for a variety of sample matrices such as tissue, blood, seeds, grains, plant materials.