W350
Fungal SM Genome Biology: How to Translate Whole Genome Sequence Information into the Secondary Metabolome in Filamentous Fungi?

Date: Sunday, January 11, 2015
Time: 11:10 AM
Room: Royal Palm Salon 3-4
Cheng-Cang Charles Wu , Intact Genomics, Inc., St Louis, MO
Jin Woo Bok , University of Wisconsin at Madison, Madison, WI
Jessica C Albright , Northwestern University, Evanston, IL
Rosa Ye , Intact Genomics, Inc., St. Louis, MO
Anthony W Anthony W Goering , Northwestern University, Evanston, IL
Neil L Kelleher , Northwestern University, Evanston, IL
Nancy P Keller , University of Wisconsin at Madison, Madison, WI
With thousands of fungal genomes being sequenced, each genome containing up to 100 secondary metabolite (SM) clusters 30 - 80 kb in size, breakthrough techniques are needed to characterize this SM wealth. We have developed a novel system-level methodology for unbiased cloning of intact SM clusters from each of 6 fungal genomes for one-step transformation and expression in a model host. All intact SM clusters from 6 species of Aspergillus, Fusarium and Penicillin were individually captured in self-replicating fungal artificial chromosomes (FAC) containing both E. coli F replicon and an Aspergillus autonomously replicating sequence. Candidate FACs were successfully shuttled between E. coli and the heterologous expression host A. nidulans.  We will present the discovery of fungal metabolome and discuss the fungal SM genome biology. 
Back to: Fungal Genomics
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