Analysis of Genetic Stability in Some Cyclamen Species Endemic to Turkey Following Cryopreservation By Vitrification
Date: Monday, January 12, 2015
Room:
Tolga Izgu
,
Department of Plant Sciences, University of California, Davis, CA
Basar Sevindik
,
Department of Horticulture, Faculty of Agriculture, University of Cukurova Balcalı, Adana, Turkey
Ozhan Simsek
,
Department of Horticulture, Faculty of Agriculture, University of Cukurova, ADANA, Turkey
Pembe Curuk
,
Department of Horticulture, Faculty of Agriculture, University of Cukurova, Adana, Turkey
Yildiz Kacar
,
CUKUROVA UNIVERSITY, ADANA, Turkey
Yesim Yalcin Mendi
,
Department of Horticulture, Faculty of Agriculture, University of Cukurova, ADANA, Turkey
The origin of 20 Cyclamen taxons, belonging to Primulaceae and growing under trees and bushes, is Mediterranean region. Ten Cyclamen species grown naturally in our country, five of which are endemic to Turkey Cyclamens are under extinction due to destruction of natural habitats, unconscious usage of agricultural areas and taking out the tubers continuously from nature for export. For this reason, conservation studies and biotechnological researches on this important genetic resource are quite significant.
In vitro preservation possibilities of Cyclamen (C. cilicium, C. mirabile, C. parviflorum and C. pseudibericum) which is an important genetic resource in our country were investigated in this study. In cryopreservation period, different osmotic reagents (mannitol, sorbitol and sucrose) and different temperatures were treated with PVS-2 and genetic stability of obtained plantlets was tested using RAPD primers. The best cryopreservation result was obtained from the treatment; 0,5 M sucrose+MS basal medium (hormone free), 48 hours, +4°C, darkness and PVS-2 solution at 0 C°, 60 min. for all species. This study showed that cryopreservation protocol worked effectivelly because the plantlets from for Cyclamen species had same genetic structure after cryopreservation. It was determined that there was no somaclonal variation between the plantlets obtained after cryopreservation and control plants using RAPD markers.
