P0816
Assessing and Validating the Amphidiploid Genome of Brassica napus Using Genotyping By Sequencing

Date: Monday, January 12, 2015
Room:
Philipp Emanuel Bayer , University of Queensland, Brisbane, Australia
Kenneth Chan , University of Queensland, Brisbane, Australia
Agnieszka Golicz , University of Queensland, Brisbane, Australia
Pradeep Ruperao , University of Western Australia, Brisbane, Australia
Paula Martinez , University of Queensland, Brisbane, Australia
Satomi Hayashi , University of Queensland, Brisbane, Australia
Yan Long , National Key Lab. of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China
Jinling Meng , National Key Lab. of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China
Tim Sutton , South Australian Research and Development Institute, Urrbrae, Australia
Boulos Chalhoub , URGV-INRA, Evry, France
Jacqueline Batley , University of Western Australia, Perth, Australia
David Edwards , University of Western Australia, Perth, Australia
Brassica napus is an economically important oilseed crop with a complex amphidiploid genome, with triplication in each of the diploid progenitors. A draft assembly of cultivar Darmor was recently published which contains pseudomolecules as well as contigs which could not be accurately placed in the genome assembly. Using high resolution skim genotyping by sequencing of a segregating population, we have been able to validate the published assembly and place additional contigs, improving the length of pseudo-chromosomes in the Darmor assembly. Using this improved assembly as a scaffolding reference, together with the population data we assembled the genome of a second B. napus cultivar Tapidor. Here we present approach for genome validation and assessment as well as a comparison of both B. napus genomes.
Back to: Genome Mapping, Tagging & Characterization: Brassicas, Arabidopsis - Even
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