Rapid and qPCR Ready DNA Isolation from Environmental and Human Bodily Fluid Samples using Magnetic Bead System

A magnetic bead system for DNA isolation was tested with the aim of producing a general approach for qPCR-ready DNA from environmental and human bodily fluid samples that are challenging to gain high quality of DNA and time consuming.  Optimization of lysis, binding and wash conditions were  done using several types of tissue samples (Soil, Stool, Saliva and Plasma). In all cases, DNA suitable for PCR was prepared in less than 30 minutes except stool and soil samples that were mechanically homogenized to gain a better DNA yield prior to the magnetic bead binding step.  Optimized washing condition removed the PCR inhibitors efficiently and DNA was simply eluted non-EDTA contained elution buffer such as Nuclease-free water.  This system is robust and reproducibly yields qPCR-ready DNA. Since there are no centrifugation steps or vacuum steps required whole DNA isolation procedure can be done efficiently in a small space.  A robotic manipulation system for DNA bound to magnetic beads is also in progress adapting an automation of DNA extraction system for the types of material tested here.