Using DNASTAR’s Lasergene Genomics Suite for Comparative Analysis of Bovine Transcriptome Sequence Data

DNASTAR offers an integrated suite of software for assembling and analyzing sequence data from all major next-generation sequencing platforms supporting key workflows on both a desktop computer as well as the DNASTAR Cloud. Our RNA-Seq alignment and analysis workflow employs the DNASTAR SeqMan NGen, SeqMan Pro, and ArrayStar applications, all components of the Lasergene Genomics Suite.  Here, next-gen RNA reads from Jersey, Holstein, and Cholistani cattle are aligned against the Bos taurus reference sequence in SeqMan NGen. Next, visualize the three samples and perform SNP analysis in SeqMan Pro. SeqMan Pro additionally offers the ability to look at the alignment of the reads to the reference and analyze what reads map to different isoforms or exons to explore differences in splice patterns. Finally, we can import the three experiments into ArrayStar for multi-sample analysis where advanced filtering options can be utilized for large-scale gene expression analysis. Innovative algorithms and highly interactive views within these three software applications offer a powerful, integrated, and user-friendly alignment and analysis workflow for RNA-Seq data.