High Quality Short Read Assembly of B10 Cucumber Line Genome

Having very broad application, nucleic acid sequencing is one of the most important tools for biological research. Four generations of high-throughput DNA sequencing technologies can be distinguished by their nature and the kind of output they provide. Till now there is no perfect method for read assembly to reconstruct the original sequence and many genome drafts are still being improved. We present second version of B10 cucumber line draft genome sequence. Originating from Eastern Himalayas and secondary domestication regions, cucumber (Cucumis sativus L.) is a widely cultivated crop. B10 is a highly inbred (20 generations) monoecious line of the Borszczagowski cucumber, an old field cultivar. Its genome was assembled by us with 80x coverage Illumina 100nt paired-end (~100nt insert) reads and BES sequences using Celera Assembler. This resulted in great, 2-fold improvement in contig’s and scaffold’s statistics comparing to previous version for which a 454 reads and the BES sequences were used. Our new assembly indicates significant role of high quality short readsin eukaryotic genome assembly.Acknowledgements: This research was supported by National Science Center (project No.NN302083139).