The Genome Survey of 11 Wild Fish Species to get Genomic Information for Diversity Assessment

With the rapid development of genome analyses and decrease in cost, genomic approaches have become available for the non-model, wild species of which genetic or genomic resources and informations are very limited. As a first step to understand genetic and genomic features for conservation of endangered wild species, we have conducted genome survey along with transcriptome for 11 fish species encompassing 6 families. More than 20X genomic sequences were used to estimate genome size and complexity. The genome size was ranged from 685Mbp to 2.6Gbp according to the different taxa with GC contents 36.3~42.2%. K-mer estimation graph suggested genetic homogeneity except for the species with taxonomic controversy. Transcriptome sequences of the combined RNA from several different tissues, i. e. fins, liver, muscle, and gonad, were de novo assembled to generate about 70,000~143,000 contigs. About 48~57% of these had blast hits, which were subsequently annotated with Gene ontology. Mitochondrial genomes were assembled from genomic sequences, of which size was ranged from 16,539bp to 17,788bp having typical mitochondrial gene contents and order. Repetitive sequences such as retrotransposons, DNA transposons, and simple sequence repeats were also cataloged. These data will be highly valuable informations for further development of molecular markers determining genetic diversities among and within populations.