2879
Lucigen - Closing and Finishing Genomes and BACs with Long Span NGS Reads

Lucigen’s long span NGS read technology is capable of generating mate pair sequencing reads that span 50 Kb in length, enabling de novo genome assemble, closure and finishing on the Illumina or Ion Torrent platforms. User defined 2-8 Kb mate pair (MP) or 10-25 Kb MP libraries can be produced with bead based or gel purification protocols, and a novel fosmid based approach generates 35-50 Kb MP libraries. This workshop introduces a new tool for long span NGS reads, with presentations demonstrating the efficient assembly, closing and finishing of genomes and BACs.
Date: Tuesday, January 13, 2015
Time: 1:30 PM-3:40 PM
Room: Pacific Salon 1
Organizer:
Curtis Knox
1:30 PM
Introduction to Lucigen's Long Span NGS Read Technology
Curtis Knox, Lucigen Corporation

1:45 PM
Closing and Finishing the Mitochondrial and Chloroplast Genomes of Sorghum Male-sterile A1 Cytoplasm using Long Span NGS Read Technology
Robert R. Klein, USDA-ARS, Southern Plains Agricultural Research Center

PDF file
2:10 PM
Construction and analysis of a Miscanthus sinensis gene space assembly
Kankshita Swaminathan, Energy Biosciences Institute

2:35 PM
Mate Pair Libraries for Enhancing Wheat BAC Assemblies
Miroslav Valarik, Institute of Experimental Botany

PDF file
3:00 PM
100 Kb Long Span Reads and Beyond
David Mead, Lucigen Corporation

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