Linkage Mapping of Peroxidase Genes in Lentil

Lentil (Lens culinaris Medic.) is one of the oldest crops in Mediterranean region with an economic importance because of the source of dietary protein in both the human diet and animal feed.

Peroxidase enzymes (POX) are conservative oxydoreductase enzymes found in plants, animals and microorganisms. Plant POX enzymes catalyzes many important reactions such as plant cell wall lignification, salt resistance, auxin degredation, formation of cell wall proteins. The enzymes also protect the plant against pathogens. As a result of these properties, POX enzymes are accepted as model enzymes in molecular mechanisms that take a role in plants’ defense systems.  The aim of this study is to find identify the location of POX genes on lentil genome.

Recombinat Inbreed Lines of LR39 were used as mapping population. Because of the conservation of POX genes, 14 primers of POX genes generated from rice and Arabidopsis genome were screened in parents of LR39. Out of 14 primers, ten primers showed polymorphism between parents. Polymorphic markers were mapped with 1939 SNP, 31 SSR markers. Among the POX markers polymorphic between parents, 6 POX markers were be able to be mapped by using Joinmap4.0 software. The markers located on LG2, LG8 and LG10. Defining the locus of the POX genes on the chromosomes will be useful for map-based cloning studies and marker assisted selection because POX has important role in plants defense systems.