Construction of Linkage Map Through Genotyping by Sequencing in Lentil (Lens culinaris Medic.)

Lentil (Lens culinaris Medic.), is a diploid (2n = 2x = 14), self-pollinating crop with a genome size of 4063 Mbp. It is a rich source of protein in both the human diet and animal feed. High density linkage mapping is necessary to define precise location of genes and QTLs. Recently Elshire et al (2011) developed technique to identify large amount of SNPs. As s frame of this project, recombinat inbreed lines of LR39 was used as a genetic materials. A total of 5355 SNPs were developed using GBS technique. After filtering the data 1939 SNP were subjected to mapping analysis. A total of 10 linkage groups (8 major and 2 minor) were obtained.

SNP s were equally distributed throughout genome. The LGs that contained high number of SNPs were LG5, low number of SNPs was LG10. Average distance between adjacent markers was 0.63 cM. Total length of the map was 609.3 cM. The longest LG was 148.4 cM and the shortest LG was 7.2 cM. The population of recombinant inbred lines produced in this study showed high level of polymorphism between parents. GBS markers with the recent advances in next generation sequencing technologies allow us to reduce distance between adjacent markers, though the technique allowed us construct high-density linkage map.

This linkage map could be used for lentil molecular breeding. It can be used for identification of QTL regions linked to agronomic traits, and for marker-assisted selection in lentil.