Xiaohan Yang
,
Oak Ridge National Laboratory, Oak Ridge, TN
Rongbin Hu
,
Oak Ridge National Laboratory, Oak Ridge, TN
John C. Cushman
,
Department of Biochemistry and Molecular Biology, University of Nevada, Reno, Reno, NV
Jerry Jenkins
,
DOE Joint Genome Institute, Huntsville, AL
Jeremy Schmutz
,
Hudson Alpha, Huntsville, AL
Gerald A. Tuskan
,
Oak Ridge National Laboratory, Oak Ridge, TN
Hengfu Yin
,
Chinese Academy of Forestry, Fuyang, China
Shengqiang Shu
,
DOE Joint Genome Institute, Walnut Creek, CA
Daniel S. Rokhsar
,
DOE Joint Genome Institute, Walnut Creek, CA
David M. Goodstein
,
DOE Joint Genome Institute, Walnut Creek, CA
Uffe Hellsten
,
DOE Joint Genome Institute, Walnut Creek, CA
Anne M. Borland
,
Newcastle University, Newcastle Upon Tyne, United Kingdom
Ray Ming
,
University of Illinois at Urbana-Champaign, Urbana, IL
Robert VanBuren
,
Danforth Plant Science Center, St Louis, MO
Ching Man Wai
,
University of Illinois at Urbana-Champaign, Urbana, IL
Haibao Tang
,
University of Arizona, Tucson, AZ
Paul E. Abraham
,
Oak Ridge National Laboratory, Oak Ridge, TN
Hao-Bo Guo
,
University of Tennessee, Knoxville, TN
Rebecca L. Albion
,
Department of Biochemistry and Molecular Biology, University of Nevada, Reno, Reno, NV
Travis Michael Garcia
,
Department of Biochemistry and Molecular Biology, University of Nevada, Reno, Reno, NV
Jungmin Ha
,
Department of Biochemistry and Molecular Biology, University of Nevada, Reno, Reno, NV
Sung Don Lim
,
Department of Biochemistry and Molecular Biology, University of Nevada, Reno, Reno, NV
Jesse A. Mayer
,
Department of Biochemistry and Molecular Biology, University of Nevada, Reno, Reno, NV
Won Cheol Yim
,
Department of Biochemistry and Molecular Biology, University of Nevada, Reno, Reno, NV
Bernie Wone
,
University of South Dakota, Vermillion, SD
Daniel Jacobson
,
Oak Ridge National Laboratory, Oak Ridge, TN
Deborah Weighill
,
Oak Ridge National Laboratory, Oak Ridge, TN
Xueqiong Li
,
Northern Illinois University, DeKalb, IL
Yanbin Yin
,
Northern Illinois University, DeKalb, IL
James Hartwell
,
University of Liverpool, Liverpool, United Kingdom
Jim Leebens-Mack
,
University of Georgia, Athens, GA
Andrew Smith
,
Oxford University, Oxford, United Kingdom
Klaus Winter
,
Smithsonian Tropical Research Institute, Balboa, Panama
Crassulacean acid metabolism (CAM) is a water-conserving photosynthetic pathway. CAM has evolved multiple times in a wide range of vascular plants. However, the molecular basis of CAM evolution is not well understood. The newly available CAM genomic resources provide an excellent opportunity for comparative analysis of CAM evolution in multiple plant lineages. In this study, we conducted genome-wide analysis of positive selection in protein-coding sequences in two CAM species, Kalanchoë laxiflora (representing an eudicotyledonous lineage), and Ananas comosus (pineapple; representing a monocotyledonous lineage) in comparison with C3 plant species. We compared day-night courses of gene expression between CAM and C3 plant genes for thousands of orthologous groups. We also performed an in-depth analysis of selected gene families relevant to CAM physiology. Our results highlight the role of positive selection and diel re-programming of gene expression in CAM evolution. This study provides important novel insights into the molecular mechanism underlying CAM evolution.
