Exploring Genes Resistant to Cotton Bollworm in Wild Soybean

Cotton bollworm (Helicoverpa armigera) is one of damaging pests for soybean (Glycine max). Identification and utilization of native resistance genes in soybean are one of economical and environmentally means to control this pest. Wild soybean (Glycine soja), YD59, was highly resistant to cotton bollworm in comparison with cv. Tianlong 2, PI535807 and PI 533604 in either choice or no-choice assays. In order to reveal the resistance mechanism, we conducted transcript and protein profiling for YD59 and a susceptible cultivar, 05-4, at 24h after inoculation by Cotton bollworm. The results showed that 2073 transcripts and 119 proteins were specifically regulated in YD59, which included protein kinases, transcriptional factors and genes involved in hormone biosynthesis and signal transduction. Noticeable, a large number of genes related to anti-nutritive or toxic proteins were specifically induced in ED059. Functions of three genes, #33, #34 and #35, were confirmed by transgenic studies in Arabidopsis. Currently, QTL mapping of resistance gene in YD59 were ongoing in our lab.