Proteogenomic Integration for Mitochondrial Dysfunction in Chicken DF-1 and LMH Immortal Cell Lines

Continuously growing immortal chicken cell lines including immortal DF-1 chicken embryo fibroblast (CEF) and LMH (leghorn male hepatoma) have been widely used for research areas of virology, cell biology, and molecular biology in avian species. The DF-1 line, which was derived spontaneously from primary CEF cells, and the LMH line, which was chemically induced, have been widely used for the propagation of avian infectious viruses including avian influenza, avian infectious bronchitis virus, Marek’s disease virus serotype 1 (MDV-1), avian metapneumovirus (AMPV), and infectious laryngotracheitis virus (ILTV). Earlier studies with primary cells, DF-1 and LMH cell lines showed various differences in the mechanisms of cell cycle regulation, telomerase activities and mitochondrial functions in response to oxidative stresses. Using RNAseq, shotgun proteomics and bioinformatic pathway analysis, functionally integrated pathways were characterized in primary CEF, DF-1 CEF and LMH cells to regulate various cellular mechanisms including mitochondrial dysfunctions in this study.