An Update on Sequencing the Cabernet Sauvignon Genome / Towards a Grapevine Information System

Sequencing of Clone 8 of Cabernet Sauvignon (Vitis vinifera L.) was initiated in 2011. Several different stages with different technologies have taken place since then. At an earlier stage, an assembly of the genome was attempted with approximately 110x Illumina reads and 5x PacBio reads. Different assemblies were compared for denovo assembly using SOAPdenovo2,  and with a hybrid approach using SPAdes V3.0, Celera WGS8.1, and SSPace-longreads.  The longer sequencing reads from PacBio made major improvements in the assembly compared to the SOAPdenovo2 results with Illumina reads only. However, the assembly results were still unsatisfactory, so an additional 100x genome coverage with PacBio RS II chemistry have been generated. An update on the current sequencing results and status of the assembly will be presented./In the frame of the IGGP, a strategy for the development of a grapevine international information system has been achieved and has now to be implemented into a road map. A presentation of the strategy will be made for discussion with a larger community.