P0162Global Insights into the Geminivirus and Potyvirus Interaction with Nicotiana benthamiana at the Transcriptome Level
Global Insights into the Geminivirus and Potyvirus Interaction with Nicotiana benthamiana at the Transcriptome Level
Plant-virus interactions are characterized by changes in host gene expression. Plant gene expression changes can reflect active responses to counteract virus infection which can be either virus specific or, a general response to biological stress.
This research focuses the question how viruses manipulate their host. The RNA virus Cassava brown streak virus (CBSV) and the ssDNA virus African cassava mosaic virus both with distinct plant invasion characters and replication strategies were studied. A RNA-seq time course (6, 12,19 dpi) study was conducted monitoring gene expression in the model plant N. benthamiana. At 12dpi, virus content was found similar for both viruses hence this time point was chosen. After normalization and calculating the logarithmic fold change as well as filtering of data (pvalue < 0.1) we identified 30-38% differentially expressed genes (DEG) were identified with significantly more differences in plants infected with CBSV compared to ACMV. Altered transcripts involved in several gene ontology categories; primary metabolite processes, localization, cellular biosynthesis were similar in both disease processes while transferase and catalytic activities differed significantly between the viruses. KEGG pathway analysis showed that energy metabolism, signal transduction, transcription, folding, sorting and degradation mechanisms were influenced similarly. In contrast replication and repair, terpenoids and polyketide metabolism were more strictly regulated in response to ACMV while infection with CBSV resulted in altered carbohydrate and amino acid metabolism.
This study clarifies transcriptome modification during infection processes by identifying common and specific responses to DNA and RNA viruses, and highlights the correlation between symptom severity and transcriptional changes in a susceptible virus/host interaction.
This research focuses the question how viruses manipulate their host. The RNA virus Cassava brown streak virus (CBSV) and the ssDNA virus African cassava mosaic virus both with distinct plant invasion characters and replication strategies were studied. A RNA-seq time course (6, 12,19 dpi) study was conducted monitoring gene expression in the model plant N. benthamiana. At 12dpi, virus content was found similar for both viruses hence this time point was chosen. After normalization and calculating the logarithmic fold change as well as filtering of data (pvalue < 0.1) we identified 30-38% differentially expressed genes (DEG) were identified with significantly more differences in plants infected with CBSV compared to ACMV. Altered transcripts involved in several gene ontology categories; primary metabolite processes, localization, cellular biosynthesis were similar in both disease processes while transferase and catalytic activities differed significantly between the viruses. KEGG pathway analysis showed that energy metabolism, signal transduction, transcription, folding, sorting and degradation mechanisms were influenced similarly. In contrast replication and repair, terpenoids and polyketide metabolism were more strictly regulated in response to ACMV while infection with CBSV resulted in altered carbohydrate and amino acid metabolism.
This study clarifies transcriptome modification during infection processes by identifying common and specific responses to DNA and RNA viruses, and highlights the correlation between symptom severity and transcriptional changes in a susceptible virus/host interaction.