P0478
Candidate genes for ESC Disease Resistance of Catfish as Revealed by a Genome Wide Association Study

Tao Zhou , Auburn University; The Fish Molecular Genetics and Biotechnology Laboratory, Auburn, AL
Shikai Liu , Auburn University; The Fish Molecular Genetics and Biotechnology Laboratory, Auburn, AL
Xin Geng , Auburn University; The Fish Molecular Genetics and Biotechnology Laboratory, Auburn, AL
Chen Jiang , Auburn University; The Fish Molecular Genetics and Biotechnology Laboratory, Auburn, AL
Yulin Jin , Auburn University; The Fish Molecular Genetics and Biotechnology Laboratory, Auburn, AL
Xiaozhu Wang , Auburn University; The Fish Molecular Genetics and Biotechnology Laboratory, Auburn, AL
Ning Li , Auburn University; The Fish Molecular Genetics and Biotechnology Laboratory, Auburn, AL
Suxu Tan , Auburn University; The Fish Molecular Genetics and Biotechnology Laboratory, Auburn, AL
Lisui Bao , Auburn University; The Fish Molecular Genetics and Biotechnology Laboratory, Auburn, AL
Jun Yao , Auburn University; The Fish Molecular Genetics and Biotechnology Laboratory, Auburn, AL
Yu Zhang , Auburn University; The Fish Molecular Genetics and Biotechnology Laboratory, Auburn, AL
Jiaren Zhang , Auburn University; The Fish Molecular Genetics and Biotechnology Laboratory, Auburn, AL
Luyang Sun , Auburn University; The Fish Molecular Genetics and Biotechnology Laboratory, Auburn, AL
Jianbin Feng , Auburn University; The Fish Molecular Genetics and Biotechnology Laboratory, Auburn, AL
Ludmilla Kaltenboeck , Auburn University; The Fish Molecular Genetics and Biotechnology Laboratory, Auburn, AL
Zhanjiang Liu , Auburn University; The Fish Molecular Genetics and Biotechnology Laboratory, Auburn, AL
Enteric septicemia of catfish (ESC), caused by the bacterial pathogen Edwardsiella ictaluri, is the leading disease problem for the catfish industry. It causes tens of millions of dollars of economic losses each year. Channel catfish (Ictalurus punctatus) is generally susceptible to the disease while blue catfish (Ictalurus furcatus) is generally resistant. Therefore, the hybrid catfish is a great model system to study ESC resistance because of the strong phenotypic contrast between channel catfish and blue catfish. In this study, 499 individuals of the fourth generation of backcross progenies of hybrid catfish were used to conduct genome wide association study (GWAS) for ESC resistance. A genomic region on linkage group 1 was found significantly associated with ESC disease resistance. Within this region, 14 genes have known functions in immunity, including wwp1, inhba, fzd8, klf6, nck1, agtr1, racgap1, nlrp12, trpc1, acbd5a, apbb1ip, myo3a, stat2, nlrc3. Among the 14 genes, 8 genes or their paralogs were found differently expressed between ESC resistance and sensitive catfish by RNA-seq analysis of previous study. The genes within the significant QTL on LG1 were known to be related to immune functions, such as phagocytosis, defense response, stimulus response, T cell differentiation, B cell differentiation, macrophage development, antigen processing and presentation. In addition to the significant QTL, two suggestively associated QTL regions were identified on linkage group 12 and 16. It appears that the identified QTLs are limited to specific families of F4 fish, consistent with the segregation of the genomic regions derived from the original blue catfish parent.
Back to: Genome Mapping, Tagging & Characterization: Aquaculture
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