TBGlobal Analysis of the RNA Secondary Structure and RNA-Protein Interaction Landscapes of Plants

At the heart of post-transcriptional regulatory pathways in eukaryotes are cis- and trans-acting features and factors including RNA secondary structure as well as RNA-binding proteins (RBPs) and their recognition sites on target RNAs. This is especially evident for RNA molecules whose functionality, maturation, and regulation requires formation of correct secondary structure and RNA-protein interactions. However, the global influence of these features on plant gene expression is still largely unclear. We have recently developed a high-throughput sequencing based approach that allows a simultaneous view of the RNA secondary structure and RNA-protein interaction site landscapes transcriptome-wide in eukaryotes. We have used this approach on multiple plant species and during their responses to various conditions and treatments. Our most recent findings from these studies will be presented.