Global DNA Methylation in Vermont Bobcat (Lynx rufus) Liver

Bobcats (Lynx rufus) are at an increased risk for mortality within the next 20 to 30 years due to habitat loss and fragmentation in Vermont. Research has focused on the conservation of bobcat using genetics and ecological population structure.  However, the use of epigenetic effects for conservation purposes is novel. This study investigates the effects of global DNA methylation in the liver of 30 Vermont bobcat. Liver samples were obtained from the Vermont Fish and Wildlife and DNA was extracted using phenol chloroform. The percent of global DNA methylation was quantified and calculated using the MethylFlash^TM Methylated DNA 5-mC Quantification Kit from Epigentek (Farmingdale, NY). Age, sex, weight, and location data were collected and correlated to percent global DNA methylation. The presence of differential methylation has the potential to provide insight into bobcat conservation within the different regions of Vermont. This study will lay the foundation for future research regarding the use of epigenetics as a mechanism for conservation.