How to Solve Plant Genome Complexity? A Strategy to Focus on Specific Genomic Regions of Interest

Plant genomes are complex regarding many aspects: genome size, polyploidy and the high percentage of repetitive elements. Next-generation DNA sequencing technologies (NGS) have revolutionized the genomic research by offering the capacity to obtain large amount of sequences in a short time. However, this approach is not sufficient to decipher the high complexity of plant genomes and Bacterial Artificial Chromosome (BAC) libraries are still invaluable tools for plant genome analysis. In this poster we aim at better characterize genomic regions using low coverage BAC library approach and NGS. We developed a new strategy using low coverage BAC libraries in order to efficiently focus on genomic region of interest. This strategy reduces BAC clones handling steps to isolate the most interesting region. Apart from this, we can now obtain high quality DNA sequences using long read sequencing technologies. In this poster we discuss the interest of combining these two technologies to explore the diversity of various plant species (a study case will be presented). This approach is an efficient way to perform comparisons of various genotypes by decreasing time and cost of library construction.