CLC Microbiome Genomics Module: Interactive Metagenome Analysis Tools

Next generation sequencing has enabled to study a range of new applications. Metagenome analysis is one of those applications. Now researchers are able to examine sample from a variety of resources such as soil, river water and gut microbiome. There are several analysis tools however those tools are not easy to use for everyone. Therefore interactive and intuitive tool can overcome the difficulties. We created CLC Microbial Genomics Module (MGM) that is metagenomics analysis module for CLC Genomics Workbench. The module includes standard analysis pipelines that include quality check, trimming, alignment to greengens, alpha and beta diversity, adding metadata and PCoA anlsysis. MGM provides interactive viewer that will be helpful to interpret the result and possible to add metadata. Metadata can be added with multiple formats. We present analysis results using publically available chicken gut microbiome data that genomic DNA was extracted from two different parts of the intestines (ilea and caeca) with different ages (7, 14, 21 and 42 days-old). The analysis included alpha diversity, bar charts with different level of taxonomy, PCoA analysis. With these analysi we will show how these results was obtained by implemented analysis pipeline in details.