A Survey of Protein Folds into the Ataulfo Mango Transcriptome

Using the Ataulfo mango RNAseq transcriptome (see talk at the Mango Genomics workshop), we classified the amino acid deduced sequences for protein fold families. We are interested in identifying novel folds and proteins that contribute to understand structure and function. For example, we have crystallized and solved the crystal structure of mango GST which is 50% identical to wheat GST (PDB 1GWC) and there is a root mean square deviation (RMSD) of 1.6 Å between both structures. We are using Phyre2 algorithm to predict structure into the clean classified transcriptome Ataulfo mango transcriptome. We surveyed the triose phosphate isomerase fold as well as the cell wall modifying enzymes. This on-going project is an example of a fruitful collaboration between agri-genomics and structural biology to identify novel targets and also to understand structure and function relationships in proteins.