P1041A Draft Mitochondrial Genome for Leucaena trichandra (Mimosoidaea: Leguminosae)
A Draft Mitochondrial Genome for Leucaena trichandra (Mimosoidaea: Leguminosae)
Mitochondrial genomes are essential for the survival of eukaryotic cells, providing critical genetic information for the production of ATP. However, as a result of high rates of intracellular recombination, few plant mitochondrial genomes have been sequenced so far. This is even true for the ecologically and economically important legumes, where mitochondrial genome sequences are only available for five closely related papilionoid taxa. To diversify the representation of available legume mitochondrial genomes, we generated a draft sequence assembly for the Leucaena trichandra, a species belongs to Mimosoidaea family. The assembly process involved mapping PacBio reads to the soybean and cotton mitochondrial reference genomes to gather seed sequences for a self-corrected PBcR-Celera assembly. The resulting contigs were further assembled in Geneious and scaffolded with the complete set of PacBio reads using SS-SpaceLongReads. The final draft assembly resulted into four contigs totaling over 800kb. The preliminary mitofy annotation recognized 40 of 41 protein coding genes and 29 of 31 tRNA genes typically found in any plant mitochondria.