Hélène Rimbert
,
INRA GDEC, Clermont-Ferrand, France
Ambre-Aurore Josselin
,
INRA GDEC, Clermont-Ferrand, France
Benoît Darrier
,
INRA GDEC, Clermont-Ferrand, France
Pierre Sourdille
,
INRA GDEC, Clermont-Ferrand, France
François Balfourier
,
INRA GDEC, Clermont-Ferrand, France
Valerie Barbe
,
CEA - Genoscope, Evry, France
Adriana Alberti
,
CEA - Genoscope, Evry, France
Karine Labadie
,
CEA - Genoscope, Evry, France
Sophie Mangenot
,
CEA - Genoscope, Evry, France
Arnaud Couloux
,
CEA - Genoscope, Evry, France
Patrick Wincker
,
CEA - Genoscope, Evry, France
Arnaud Bellec
,
INRA - CNRGV, Castanet Tolosan, France
Hélène Bergès
,
INRA - CNRGV, Castanet Tolosan, France
Michael Alaux
,
INRA - URGI, Versailles, France
Thomas Letellier
,
INRA - URGI, Versailles, France
Hadi Quesneville
,
INRA - URGI, Versailles, France
Zeev M. Frenkel
,
Institute of Evolution, University of Haifa, Haifa, Israel
Tzion Fahima
,
Institute of Evolution, University of Haifa, Haifa, Israel
Abraham B. Korol
,
Institute of Evolution, University of Haifa, Haifa, Israel
Etienne Paux
,
INRA GDEC, Clermont-Ferrand, France
We are currently producing a reference sequence of the bread wheat chromosome 1B (535 Mb) by sequencing 6023 BAC clones comprising the minimal tilling path of the physical maps of both short and long arms. BACs were sequenced by pools using a combination of Illumina MiSeq paired-end reads and HiSeq2500 5 kb mate pairs. First round of assembly yielded 7131 scaffolds representing 534 Mb. Report on the improvement of this assembly, the construction of a pseudomolecule and the comparison with the previous sequencing and assembly of chromosome 3B will be presented.
