Getting on Track: DNA Barcoding Projects As a Vehicle to Engage Community College Students in Basic Life Sciences Research.

We have developed the “Sequencing to Success DNA Barcoding Challenge,” a module based on DNA barcoding that introduces students to the process of science from developing a question, experimentation, data analysis, and presentation of results. While similar to the module developed by the Dolan DNA Learning Center, we have targeted community college transfer students and high school teachers. These participants have limited time to spend in a research facility so we do the DNA analysis and poster preparation using interactive online platforms. The module begins with the question “Is the fish at the store properly labeled?” or “What plant is this?” Participants bring their sample to the lab. During their visit, students extract DNA from the sample, perform PCR using 16S (fish) or RBCL (plants) primers and analyze the results on agarose gels. During down time the students learn to use the electronic notebook (e-LN) platform and Google Drive where data will be exchanged and discussed. During the next week, the PCR products are sequenced and the results are shared electronically. Over the course of several weeks, students work with instructors in person or through Skype/Google-Hangouts to learn the DNA Subway for data analysis. Participants write an abstract that is reviewed and corrected by the instructors. Lastly, students prepare a poster for a symposium at UCR three to four weeks after the laboratory work. This program has served ninety-six community college students from Riverside and San Bernardino Counties over three years. Protocols will be made available at the presentation.