Explore the Truth About Cats and Dogs with Affymetrix

Genotyping solutions from Affymetrix provide breeders and researchers with powerful and flexible tools to identify, screen and validate complex genetic traits in animals. These include both arrays and next generation sequencing (NGS)-based assays. Axiom® Genotyping Solution is fully automated and can process more than eight sample plates per week with a single instrument.  It allows for high throughput screening of markers of interest in 96-array format, with selection of markers of interest in 384-array format.   This platform can be used to for phenotype-trait association and selection applications. At the other end of the spectrum, many researchers need an affordable high throughput method to provide customizable genotyping for interrogation of tens to thousands of markers per sample. Eureka™ Genotyping Solution utilizes NGS to enable genotyping of thousands of DNA samples for tens to thousands of loci. The Eureka genotyping assay is a ligation-dependent PCR reaction, which uses interrogation site bar codes contained within the ligation probes, as well as sample index bar codes added during the amplification step. Next generation sequencing libraries can be created for thousands of DNA samples within 24 hours. The Eureka genotyping assay has been used to interrogate simple and poly allelic SNPs, small to large INDELs, tetra-ploids and for the detection of low percent contamination in a large background of non-target DNA. The Eureka Genotyping Solution is flexible, accurate, specific, affordable and robust.