P1015
A Multiparent Advanced Generation Inter-Cross Population for Genetic Analysis and Breeding in Cowpea (Vigna unguiculata L. Walp.)

Bao-Lam Huynh , Department of Nematology, University of California - Riverside, Riverside, CA
Jasmine L. Gracin-Dixon , Department of Nematology, University of California - Riverside, Riverside, CA
Yi-Ning Guo , Department of Botany & Plant Sciences, University of California - Riverside, Riverside, CA
Jansen R. P. Santos , Department of Nematology, University of California - Riverside, Riverside, CA
Arsenio D. Ndeve , Department of Nematology, University of California - Riverside, Riverside, CA
Maria Muņoz-Amatriain , Department of Botany & Plant Sciences, University of California - Riverside, Riverside, CA
Sassoum Lo , Department of Botany & Plant Sciences, University of California - Riverside, Riverside, CA
Ira Herniter , Department of Botany & Plant Sciences, University of California - Riverside, Riverside, CA
Steve Wanamaker , Department of Botany & Plant Sciences, University of California - Riverside, Riverside, CA
Tra T.T. Duong , Department of Nematology, University of California - Riverside, Riverside, CA
Savanah St. Clair , Department of Botany & Plant Sciences, University of California - Riverside, Riverside, CA
Mitchell R. Lucas , Department of Botany & Plant Sciences, University of California - Riverside, Riverside, CA
Jeffrey D. Ehlers , The Bill and Melinda Gates Foundation, Seattle, WA
Timothy J. Close , Department of Botany & Plant Sciences, University of California - Riverside, Riverside, CA
Philip A. Roberts , Department of Nematology, University of California - Riverside, Riverside, CA
Development and analysis of Multiparent Advanced Generation Inter‐Cross (MAGIC) populations have been conducted with several crop plants due to the potential for dissecting the genetic structure of traits and improving breeding populations. As part of the Tropical Legumes I Project (GCP-funded, 2009-2015), we developed an 8-parent MAGIC population for cowpea (Vigna unguiculata L. Walp.). The 8 founder parents (IT89KD-288, IT84S-2049, CB27, IT82E-18, SuVita 2, IT00K-1263, IT84S-2246, and IT93K-503-1) are highly diverse and carry many abiotic and biotic stress resistance, seed quality and agronomic traits relevant to cowpea improvement in sub-Saharan Africa. They were inter-mated using structured matings to ensure a population would have equal representation from each of the founder parents, followed by single-seed descent, resulting in more than 300 F8 recombinant inbred lines (RILs) each carrying a mosaic of genome blocks contributed from all founders. This was confirmed by SNP genotyping with the Illumina 60K iSelect as part of the Feed the Future Innovation Lab for Climate Resilient Cowpea. The F8 lines were on average 99.74% homozygous while also diverse in morphological traits including growth habit, flower color, flowering time, maturity, leaf shape, pod load, and seed size, shape, color and texture. Due to its broad genetic base, the cowpea MAGIC population promises breakthroughs in genetic gain, QTL discovery, and construction of high-resolution genetic maps for use in haplotype-trait definition and genome assembly.
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