Cloning and Characterization of Functional Naïve VHH Library That Can be Used in Phage Display for Selection of Antigen-Specific Nanobodies

Heavy Chain Antibodies (HCAB) found in camelids and shark are relatively newly-discovered class of antibodies that are getting increased focus for their potential use in different applications such as drug discovery and plant protection. The antigen-binding variable fragment of heavy chain HCAB (VHH; also called Nanobody) possess a unique combination of physical and chemical properties that makes it a promising target for medical and plant applications. Here we report the generation and characterization of functional naïve VHH phage library. Several nanobodies against different antigens were isolated from the naïve library using phage display technology.

Acknowledgment:  This project was funded by the National Plan for Science, Technology and Innovation (MAARIFAH), King Abdulaziz City for Science and Technology, Kingdom of Saudi Arabia, Award Number BIO2833.