07
Genome Technology: Other Genome Methodology

P0230
Targeted Sequencing of Genes and Transcripts from Soybean and Wheat using NimbleGen SeqCap EZ and PacBio SMRT Sequencing
Jenny Gu, Pacific Biosciences; Kevin Eng, Pacific Biosciences; Anand Sethuraman, Pacific Biosciences; Denise Raterman, Roche NimbleGen; Daniel Burgess, Roche NimbleGen; Todd A. Richmond, Roche NimbleGen; Steve Kujawa, Pacific Biosciences

P0231
Study on the Effect of Biological Damage in Plant Caused by Low Energy Ion Beam Implantation
Qiuxia Liang, Henan Province Key Laboratory of Ion Beam Bioengineering, Zhengzhou university; Gangqiang Cao, School of Life Sciences Zhengzhou University; Zhen Jiao, Zhengzhou University; Qunce Huang, Henan Province Key Laboratory of Ion Beam Bioengineering, Zhengzhou University

P0232
Genome-wide Annotation of Mutations in a Phenotyped Mutant Library Provides an Efficient Platform for Discovery of Causal Gene Mutations
Yinping Jiao, USDA-ARS/Cold Spring Harbor Laboratory; John Burke, USDA-ARS; Ratan Chopra, USDA-ARS; Gloria Burow, USDA-ARS; Junping Chen, USDA-ARS; Bo Wang, Cold Spring Harbor Laboratory; Chad Hayes, USDA-ARS; Yves Emendack, USDA-ARS; Doreen Ware, Cold Spring Harbor Laboratory/USDA-ARS; Zhanguo Xin, USDA ARS

P0233
plantGPS: A Whole-Genome Modeling Framework to Accurately Predict Quantitative Traits
Yuri V. Nikolsky, George Mason University; Tatiana Tatarinova, University of Southern California

P0234
Rational Design of High-Number DNA Fragments for Gene Construction
Bhagyashree S Birla, Iowa State University; Hui-Hsien Chou, Iowa State University

P0235
A Powerful Tool for Efficient Recurrent Selection: Positively and Negatively Selectable Male-Sterile Rice
Junichi Tanaka, National Agriculture and Food Research Organization; Maiko Akasaka, NARO Institute of Crop Science (NICS); Yojiro Taniguchi, NARO Institute of Crop Science (NICS); Masao Oshima, National Institute of Agrobiological Sciences (NIAS); Kiyomi Abe, National Institute of Agrobiological Sciences (NIAS); Yutaka Tabei, National Institute of Agrobiological Sciences (NIAS)

P0236
Improved Isolation Methods of HMW DNA from Plants and Animal Tissues
Boudewijn F.H. ten Hallers, BioNano Genomics; Claire Q. Yang, BioNano Genomics; Michael G. Saghbini, BioNano Genomics; Kenneth G. Sugerman, BioNano Genomics

P0237
A Novel Method for Generating Dihaploids in Tall Fescue
Bryan Kindiger, USDA-ARS, Grazinglands Research Lab; Devesh Singh, Barenbrug Seeds, USA

P0238
In-Depth Analysis of the Microbial Diversity in Saliva Samples Preserved at Ambient Temperature for over 6 years
Won-Sik KIM, Norgen Biotek Corp.; Bernard Lam, Norgen Biotek Corp.; Vanja Misic, Norgen Biotek Corp.; Yousef Haj-Ahmad, Norgen Biotek Corp.

P0239
Genome Wide Evaluation of the Impact of Targeted Gene Editing in Diploid Potato
Satya Swathi Nadakuduti, Michigan State University; Nathaniel M. Butler, Michigan State University; Courtney Leisner, Michigan State University; Colby Starker, University of Minnesota; Dan Voytas, University of Minnesota; C. Robin Buell, Department of Plant Biology and DOE Great Lakes Bioenergy Research Center, Michigan State University; David S. Douches, Michigan State University

P0240
ENVIRATRON - An Accelerator for Climate Change Research
Stephen H. Howell, Iowa State University; Lie Tang, Iowa State University; Carolyn J. Dill, Bioinformatics and Computational Biology Program, Iowa State University; Thomas Lubberstedt, Iowa State University; Steven A. Whitham, Iowa State University

P0241
Telomere-to-Telomere Chromosome Assemblies and Identification of Structural Variations in Arabidopsis thaliana Ecotypes
Florian Jupe, Salk Institute for Biological Studies & Howard Hughes Medical Institute; Todd P. Michael, IBIS Biosciences; Zhuzhu Zhang, Salk Institute for Biological Studies & Howard Hughes Medical Institute; Chongyuan Luo, Salk Institute for Biological Studies & Howard Hughes Medical Institute; Mark Zander, Salk Institute for Biological Studies & Howard Hughes Medical Institute; Joseph Ecker, Salk Institute for Biological Studies & Howard Hughes Medical Institute

P0242
Developing Protocols for Animal Genebanks : Exploiting Limited Amounts of DNA By Whole Genome Amplification
Marie-Noelle Rossignol, INRA UMR GABI; Marco Moroldo, Institut National de la Recherche Agronomique, AgroParisTech, UMR1313 Génétique Animale et Biologie Intégrative; Yves Amigues, LABOGENA DNA; Michèle Tixier-Boichard, INRA, UMR GABI

P0243
Dissecting Meiotic Recombination based on Tetrad Analysis by Single Microspore Sequencing in Maize
Xiang Li, Huazhong Agricultural University; Lin Li, University of Minnesota; Jianbing Yan, Huazhong Agricultural University

P0244
A Simple Mixer-Mill Method for Fragmentation of Genomic DNA Templates for Droplet Digital PCR
Sung-Jong Lee, Canadian Grain Commission; Daniel Perry, Canadian Grain Commission

P0245
Comparative Analysis of Single Base Resolution Whole Genome Bisulfite Sequencing Methods for Low-Input Samples
Taiji Kawakatsu, National Institute of Agrobiological Sciences; Joseph R. Nery, Salk Institute for Biological Studies; Joseph Ecker, Salk Institute for Biological Studies & Howard Hughes Medical Institute

P0246
Development and Application of a Codon-Optimized CRISPR/Cas9 System for Targeted Mutagenesis in Soybean and Medicago truncatula
Junqi Liu, Department of Agronomy and Plant Genetics, University of Minnesota; Jean-Michel Michno, Department of Agronomy and Plant Genetics, University of Minnesota; Xiaobo Wang, Anhui Agricultural University; Shaun J Curtin, Department of Plant Pathology, University of Minnesota; Robert M. Stupar, Department of Agronomy and Plant Genetics, University of Minnesota

P0247
Insertions of Mutator-Like Transposable Elements and Their Impact on Gene Expression in the Illinois Long-Term Selection Experiment Maize Strains
Dongyan Zhao, Michigan State University; Stephen P. Moose, University of Illinois, Urbana-Champaign; Ning Jiang, Michigan State University

P0248
De Novo Genome Assembly of Heterozygous Vitis species Using Next-Generation Sequencing
Sagar Patel, South Dakota State University; Padmapriya Swaminathan, South Dakota State University; Erliang Zeng, University of South Dakota; Anne Fennell, South Dakota State University

P0249
Nucleic Acid Purification From Plant Tissues for Viral Detection
Samantha Lewis, Promega Corporation; Laurence Delaurière, Promega Europe Training and Application Lab (PETAL); Becky Carroll, Promega Corporation; Elisa Ruiz, Promega Europe Training and Application Lab (PETAL); Ellyn Lepinski, Promega Corporation; Doug Wieczorek, Promega Corporation; Céline Ménager, Promega Europe Training and Application Lab (PETAL); Eric B. Vincent, Promega Corporation; Trista Schagat, Promega Corporation

P0250
Direct DNA Amplification from Plants
Sandra Nguyen, Promega Corporation; Leta Steffen, Promega Corporation; Samantha Lewis, Promega Corporation; Trista Schagat, Promega Corporation; Doug Storts, Promega Corporation

P0251
Profiling Metagenomic Communities Using Circular Consensus and Single Molecule, Real-Time Sequencing
Richard Hall, Pacific Biosciences; Steve D, Oh, Pacific Biosciences; Cheryl Heiner, Pacific Biosciences

P0252
Nucleic Acid Purification for Monitoring Economically Important Aquatic Species
Eric B. Vincent, Promega Corporation; Laurence Delaurière, Promega Europe Training and Application Lab (PETAL); Doug Wieczorek, Promega Corporation; Elisa Ruiz, Promega Europe Training and Application Lab (PETAL); Kevin Kershner, Promega Corp; Nans Bodet, Promega; Céline Ménager, Promega; Trista Schagat, Promega Corporation

P0253
Whole Genome Amplification of Single Pollen Grains for Haplotype Determination in Citrus
Sergio Pietro Ferrante, National Research Council of Italy - Institute of Biosciences and BioResources; Mikeal L. Roose, University of California Riverside

P0254
MaGuS: A Tool for Map-Guided Scaffolding and Quality Assessment of Genome Assemblies
Mohammed-Amin Madoui, CEA - Genoscope; Léo d'Agata, CEA - Genoscope; Carole Dossat, Genoscope; Jan van Oeveren, Keygene; Edwin van der Vossen, Keygene; Jean-Marc Aury, CEA - Genoscope

P0255
Enriching for the Nucleic Acid of Interest before NGS Profiling of Plant and Animal Samples
Daniela B Munafo, New England Biolabs; Erbay Yigit, New England Biolabs; Deyra N. Rodriguez, New England Biolabs; Bradley W Langhorst, New England Biolabs; Fiona J Stewart, New England Biolabs; Eileen T Dimalanta, New England Biolabs; Theodore B Davis, New England Biolabs

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