W601
Identification of Functional Variants in Soybean Using Fast Neutron and CRISPR-Based Mutagenesis

Date: Saturday, January 14, 2017
Time: 10:30 AM
Room: Golden West
Robert M. Stupar , Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN
Benjamin W. Campbell , Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN
Austin A. Dobbels , Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN
Shaun J Curtin , USDA-ARS, St. Paul, MN
Jean-Michel Michno , Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN
Adrian O. Stec , Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN
Jeffrey Roessler , Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN
Anna N. Hofstad , Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN
Suma Sreekanta , Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN
Kamaldeep S. Virdi , Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN
Yer Xiong , Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN
Fengli Fu , Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN
Thomas J. Y. Kono , Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN
Jamie A. O'Rourke , United States Dept. of Agriculture, Ames, IA
Carroll P. Vance , Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN
Seth Naeve , Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN
Gary J. Muehlbauer , Department of Plant and Microbial Biology, University of Minnesota, St. Paul, MN
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Identification and characterization of the genes and DNA sequence polymorphisms that underlie specific trait variants remains a challenge in soybean due to limitations in mutant resources and transformation capacities. We recently developed and screened a soybean fast neutron mutant population to identify mutants with alterations to plant morphology, architecture and seed composition. A combination of genome structural analysis and genetic mapping led to the cloning of two genes, one underlying an altered trichome development phenotype and another underlying a high-sucrose/low-oil seed composition phenotype. However, for most map-based cloning projects, we have difficulty validating the candidate genes that reside within a mapped interval. We have recently initiated experiments using targeted mutagenesis approaches with CRISPR/Cas9 to validate the candidate genes for specific traits. The challenges and opportunities for using this approach in soybean will be highlighted in this talk.
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