The Non-Transgenic LORE1 Insertion Mutant Collection for Legume Genetics

With more than 640,000 annotated insertions, the non-transgenic LORE1 insertion mutant resource offers knock-out alleles of the majority of active genes in the legume Lotus japonicus (http://lotus.au.dk). A detailed characterization of the LORE1 population, including insertion preferences with respect to methylation status, transcriptional effects of insertion and estimates of background mutation rates will be presented. In addition, examples of use of the LORE1 resource in characterization of nodulation signalling will be presented.

Reference:

Małolepszy A, Mun T, Sandal N, Gupta V, Dubin M, Urbański D, Shah N, Bachmann A, Fukai E, Hirakawa H, Tabata S, Nadzieja M, Markmann K, Su J, Umehara Y, Soyano T, Miyahara A, Sato S, Hayashi M, Stougaard J, Andersen SU. The LORE1 insertion mutant resource. Plant J. 2016 Jun 20. doi: 10.1111/tpj.13243. PubMed PMID: 27322352.