P0586
Putative Causative Genes Discovery and Marker Development for Male Sterility Aiming at Marker Assisted Selection Using High-Density Linkage Map Based QTL Analysis in Japanese Cedar

Kentaro Mishima , Forest Tree Breeding Center, FFPRI, Hitachi, Ibaraki, Japan
Miyoko Fukatsu-Tsubomura , Forest Tree Breeding Center, FFPRI, Hitachi, Ibaraki, Japan
Tomonori Hirao , Forest Bio-Research Center, FFPRI, Hitachi, Ibaraki, Japan
Miho Tamura , Faculty of Agriculture, Kyushu University, Fukuoka, Japan
Manabu Kurita , Kyushu Regional Breeding Office, Forest Tree Breeding Center, FFPRI, Koshi, Kumamoto, Japan
Mine Nose , Forest Tree Breeding Center, FFPRI, Hitachi, Ibaraki, Japan
So Hanaoka , Hokkaido Breeding Office for FTBC,Forestry and Forest Products Research Institute, Ebetsu, Hokkaido, Japan
Mineko Ohira , Forest Tree Breeding Center, FFPRI, Hitachi, Ibaraki, Japan
Eitaro Fukatsu , Kyushu Regional Breeding Office, Forest Tree Breeding Center, FFPRI, Koshi, Kumamoto, Japan
Yuya Takashima , Forest Tree Breeding Center, Forestry and Forest Products Research Institute, Hitachi, Ibaraki, Japan
Taiichi Iki , Tohoku Regional Breeding Office, Forest Tree Breeding Center, FFPRI, Takizawa, Iwate, Japan
Masahiro Miura , Kansai Regional Breeding Office, Forest Tree Breeding Center, FFPRI, Shouo, Okayama, Japan
Yuichiro Hiraoka , Forest Tree Breeding Center, FFPRI, Hitachi, Ibaraki, Japan
Makoto Takahashi , Forest Tree Breeding Center, FFPRI, Hitachi, Ibaraki, Japan
Hiroshi Hoshi , Forest Tree Breeding Center, FFPRI, Hitachi, Ibaraki, Japan
Atsushi Watanabe , Faculty of Agriculture, Kyushu University, Fukuoka, Japan
We constructed an EST-based high-density linkage map, and then carried out the quantitative traits loci (QTL) analysis for male sterility using an F2 population, which were come from a sib-cross between two mutant allele carriers (F1s). For the linkage map construction, approximately 70k SNPs, which were identified from ESTs collected from several tissues, were genotyped using the Axiom genotyping system. About 7k SNPs out of 70k SNPs were polymorphic between the parents . As the result of linkage analysis (threshold LOD = 10.0), a high density linkage map of 3,205 loci forming 11 linkage groups (average marker distance: 2.15 cM) spanning 1492.8cM was constructed. A significant QTL for male sterility was detected in a region of 33.4-34.5 cM in the LG9. LOD value of the QTL was 99.9-947.5. It corresponds to explaining 100% of phenotypic variation. We developed 11 markers for marker-assisted selection (MAS) based on the marker order around the QTL region.
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